Tb green premix ex taq ii (tli rnaseh plus)
WebApr 9, 2024 · DAN amplification reactions were carried out in a TB Green Premix Ex Taq TM II (Tli RNaseH Plus) Kit (Takara, Bio Inc). For each real-time qPCR reaction, three replicates were performed in a 20.0 μL system, including 2×LightCycle 10.0μL SYBR® Premix Ex Taq ™ II (Takara, Bio Inc), 0.4μL reverse primer and forward primer,1μL … WebTB Green Premix Ex Taq (Tli RNase H Plus), an intercalating dye-based qPCR master mix for real-time RT-PCR (qPCR), is recommended for most real-time PCR applications …
Tb green premix ex taq ii (tli rnaseh plus)
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WebSep 29, 2024 · TB Green Premix Ex Taq II (Tli RNaseH Plus) (Takara, Dalian, China) was used for RT-qPCR. The system was 20 µL in total and contained 10 µL of TB Green Premix, 0.6 µL of upstream and downstream primers (10 µmol/L), 0.6 μL of Rox Reference Dye II, 1.5 μL of cDNA template, and ddH 2 O to the final volume. WebTB Green Premix Ex Taq II (Tli RNaseH Plus)は、インターカレーター法によるリアルタイムPCR専用試薬である。 2×濃度のプレミックスタイプ試薬で、リアルタイムモニタ …
Web导航: 首页> 生物化学;啤酒;烈性酒;果汁酒;醋;微生物学;酶学;突变或遗传工程>用于滑液支原体鉴定的特有功能基因、核酸染料qPCR引物、试剂盒及其应用 WebReviews. TB Green Premix Ex Taq II (Tli RNase H Plus) is a reagent specifically designed for intercalator-based real time PCR using TB Green. It is supplied at a 2X concentration …
WebAdditionally, SYBR Premix Ex Taq II (Tli RNase H Plus) is supplied at a 2X concentration, enabling easy reaction assembly. The premix also contains heat-resistant Tli RNase H … WebSep 15, 2024 · Briefly, reaction was set in a 25-μL volume containing 12.5 μL of 2 × TB Green Premix Ex Taq II (Tli RNaseH Plus) Master Mix (Takara Bio, Inc.), 2 μL of cDNA or DNA template, 8.5 μL of diethylpyrocarbonate (DEPC)-treated water and 1 μL (the stock concentration 10 μM) of each primer (SVA-FP1 (5′-TGAAGTTGCGGAGAAGAT-3′) …
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WebMay 2, 2015 · The mRNA expression level was determined by SYBR Premix Ex Taq (Tli RNaseH Plus; Takara) and ABI ViiA7 Detection System (Applied Biosystems, Foster, CA, USA); the sequences of target gene-specific primers are listed in Table 2. All reactions were carried out with the following program: 30 s at 95°C, followed by 44 cycles of 95°C for 3 s … miele softtronic w 3741 kein stromWebtakara.RR420ATB Green™ Premix Ex Taq™(Tli RNaseH Plus)50 μl反应×200 次. 品名:TB Green™ Premix Ex Taq™(Tli RNaseH Plus) 规格:50 μl反应×200 次 产品描述: 本制品是采用TB Green嵌合荧光法进行Real Time PCR的专用试剂。制品中含有Real Time PCR反应的最适... miele softtronic w 3241 anleitungWebTB Green Premix Ex Taq II (Tli RNaseH Plus) is a TB Green Master Mix that effectively suppresses amplification of primer dimers and off-target products, and is therefore recommended for real-time PCR (qPCR) when high specificity is critical. Use this product when primer design has been optimized, but when an increase in specificity of the qPCR … new to twitterWebApr 1, 2024 · Each qPCR reaction was performed using the TB Green™ Premix Ex Taq™ II (Tli RNaseH Plus) (Takara, Japan) according to the manufacturer’s guidance, … miele softtronic w 4144 testWeb注:使用Thermal Cycler Dice Real Time System II(Code No. TP900/TP960)时,建议使用TB Green Premix Ex Taq II (Tli RNaseH Plus)(Code No. RR820A)。 保 存: 4℃可保存6个月。 避光保存,避免污染。 -20℃运输。 长期保存请置于-20℃。产品融解后请于4℃保存,并在6个月内用完。 miele softtronic w3245 manualWebAug 26, 2024 · After amplification, the resulting cDNA was subjected to qPCR using TB Green Premix Ex Taq II (Tli RNaseH Plus, Takara Bio Inc.); GAPDH was selected as the housekeeping gene. The expression level of each gene was calculated via the 2 −ΔΔCt method. The qPCR primers included: NRF2, 5′-TCCAAGTCCAGAAGCCAAACTGAC-3′ … miele softtronic w 4146WebOct 29, 2024 · For qPCR, diluted genomic DNA or cDNA samples were mixed with primers and TB Green Premix Ex Taq II (Tli RNaseH Plus) (TaKaRa, Japan) according to the manufacturer’s instructions. qPCR analysis was performed on LightCycler 480 II system (Roche) using the previously described conditions except setting the annealing … miele softtronic w 2241 nachfolger