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Phosphinothricin acetyltransferase

WebGene: pat Gene Source: Streptomyces viridochromogenes Product: phosphinothricin N-acetyltransferase (PAT) enzyme Function: eliminates herbicidal activity of glufosinate (phosphinothricin) herbicides by acetylation GM Trait: Glufosinate herbicide tolerance Events with gene pat WebThe present invention relates to specific herbicide combinations comprising (i) L-glufosinate and/or salts thereof and (ii) carfentrazone-ethyl in specific ratios, and to compositions comprising said herbicide combinations in these ratios. The present invention further relates to a method of producing said specific herbicide combinations and compositions …

Arsinothricin, an arsenic-containing non-proteinogenic amino acid ...

Web2.2 Preparation of the Phosphinothricin Acetyltransferase Electrochemical Immunosensor Prior to modification, the GCE was polished with 1 μm, 0.3 µm, and 0.05 µm aluminum powder sequentially on polishing paper, with sonication in double-distilled water between each polishing step. WebPPT N-acetyltransferase. acetyl-CoA + phosphinothricin <=> CoA + H (+) + N-acetylphosphinothricin. The substrate phosphinothricin is used as a nonselective herbicide and is a potent inhibitor of EC 6.3.1.2, a key enzyme of nitrogen metabolism in plants. All UniProtKB/Swiss-Prot entries referenced in this entry, with possibility to download in ... bso velux windows https://fsanhueza.com

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WebApr 11, 2024 · phosphinothricin acetyltransferase protein responsible for tolerance to glufosinate-ammonium herbicides. DP23211 corn also contains the gene that encodes for the phosphomannose isomerase protein, which is used as a selectable marker. The petition states that DP23211 corn is unlikely to pose a plant pest risk and, therefore, should WebNov 1, 1996 · The steps involved are catalyzed by the following enzymes: step 1, PPT N-acetyltransferase (N-ac-PPT); step 2, deaminases, including L-amino acid oxidase; step 3, transaminase. Step 4 presumably ... WebDonn G, Dirks R, Eckes P, Uijtewaal B (1990a) Transfer and expression of modified phosphinothricin-acetyltransferase gene from Streptomyces viridichromogenes in tomato, melon, carrot, and strawberry. Abstr VII Int Congr Plant Tissue Cell Cult Amsterdam: 176. bso viper team

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Category:Transformation of Maize Cells and Regeneration of Fertile Transgenic …

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Phosphinothricin acetyltransferase

Glufosinate Resistance Level is Proportional to Phosphinothricin ...

WebSep 10, 2016 · To examine the possibility of using the phosphinothricin N-acetyltransferase (pat) gene as a selectable marker gene, we introduced the pat genes into D. salina with particle bombardment system under the condition of helium pressure of 900 psi from a distance of 3 cm. PCR analysis confirmed that the gene was stably inserted into the cells … WebGlufosinate (also known as phosphinothricin and often sold as an ammonium salt) is a naturally occurring broad-spectrum herbicide produced by several species of …

Phosphinothricin acetyltransferase

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WebMay 26, 2024 · Phosphinothricin acetyltransferase (PAT), the target protein (Kim et al. 2010) investigated in this study, is a typical herbicide-resistant protein coded by a bar gene isolated from Streptomyces hygroscopicus … WebApr 11, 2024 · Pioneer Hi-Bred International (Pioneer) has submitted a petition (APHIS Petition Number 20–203–01p) to APHIS seeking a determination of nonregulated status for DP23211 maize (corn), which was developed using genetic engineering for insect resistance to western corn rootworm and contains the gene that codes for the phosphinothricin ...

WebApr 1, 2005 · DP23211 maize expresses an insect-specific dsRNA, an insect-active non-Bt protein, the phosphinothricin acetyltransferase (PAT) protein for tolerance to glufosinate … Web2.2 Preparation of the Phosphinothricin Acetyltransferase Electrochemical Immunosensor Prior to modification, the GCE was polished with 1 μm, 0.3 µm, and 0.05 µm aluminum …

WebDec 1, 1993 · Phosphinothricin acetyltransferase activity was detected in extracts of the T0 plants. These plants were resistant to local application of the herbicide Ignite/Basta, and the resistance was inherited in T1 plants as a single dominant locus. WebApr 1, 2005 · DP23211 maize expresses an insect-specific dsRNA, an insect-active non-Bt protein, the phosphinothricin acetyltransferase (PAT) protein for tolerance to glufosinate herbicide, and the...

WebInactivates phosphinothricin (PPT) by transfer of an acetyl group from acetyl CoA. Can also acetylate demethylphosphinothricin but not PTT or glutamate. This enzyme is an effector …

WebApr 15, 2024 · Here we demonstrate that purified PpArsN1 exhibits phosphinothricin acetyltransferase activity (Table 1). The glutamine synthetase inhibitor L-MSO is a poorer substrate compared with AST (Fig. 1d ). exchange setup.exeWebphosphinothricin-N-acetyltransferase Test. SKU: GENE-PHOS-NAT Category: Foody Safety Assays Tag: plantExtract. Related products. urinalysis test … bso victim notificationWebOct 2, 2024 · Tolerance is conferred by the enzyme phosphinothricin acetyltransferase (PAT), encoded by the pat gene from Streptomyces viridochromogenes (Herouet et al. 2005 ). This enzyme metabolizes glufosinate in transgenic plants to N -acetyl- l -glufosinate (NAG) (Müllner et al. 1993 ). bso victim advocateWebApr 11, 2024 · phosphinothricin acetyltransferase protein responsible for tolerance to glufosinate-ammonium herbicides. DP23211 corn also contains the gene that encodes for … bs owa elmshornWebAbstract. Insertion of the gene encoding phosphinothricin acetyltransferase (PAT) has resulted in cotton plants resistant to the herbicide glufosinate. However, the lower … bso vughtbso vfireWebPhosphinothricin acetyltransferase (PAT) assays confirmed the expression of the bar gene in plants obtained from PPT-resistant calli. The only exceptions were two plants obtained from the same callus that had multiple copies of the bar gene integrated into their genomes. The transgenic status of the plants was varified by Southern blot analysis. exchange setup log location