WebRecently, we cloned vacuolar and plasma membrane-localized Al-transporters, HmVALT, and HmPALT1, which are both members of the aquaporin family. However, HmPALT1 was only expressed in the sepals, indicating that a different Al-transporter should exist for absorption and long-distance transportation in the hydrangea plant. Using genetic ... WebHmVALT and HmPALT1, from hydrangea through candidate gene searching, using sequence data obtained from cDNA libraries in silico and microarray analyses, followed …

Plasma membrane-localized Al-transporter from blue hydrangea …

Web(A) Al tolerance assay of Δhsp150 yeast cells that were transformed with the candidate genes Al1–6. Each cell was grown on 2 mM Al2(SO4)3 at pH 3.5. The time course of growth of each transformant was measured by OD600 for 2 d. (B) The intracellular Al content of the Al2-transformed Δhsp150 yeast cells. The cells were exposed to different concentrations … Web(A) Al tolerance assay of amino acid replaced HmVALT transformant. Δhsp150 yeast cells that harbored HmVALT-L162T and −G196A were spotted onto LPP medium (pH 3.5) … fast track ft4250

Hamilton Valley Management

WebAug 27, 2024 · HmVALT and HmPALT1 belong to the tonoplast-intrinsic proteins (TIPs) and nodulin 26-like intrinsic proteins (NIPs) of aquaporin, respectively. Most recently, NIP1;2 was reported to be a plasma membrane-localized transporter that facilitates Al–malate transport from the root cell wall into the root symplasm . Web(A) Phylogenetic tree was generated from a ClustalW alignment of HmVALT and selected TIP amino acid sequences using TreeView 1.6.6. (B) Alignment of the HmVALT and … Web(A) Al tolerance assay of amino acid replaced HmVALT transformant. Δhsp150 yeast cells that harbored HmVALT-L162T and −G196A were spotted onto LPP medium (pH 3.5) with or without 2 mM Al2(SO4)3, and the plates were incubated at 30°C for 4 d.HmVALT-L162T transformant grew less indicating to become more Al-sensitive but that of HmVALT … frenchton nose